Extrafloral Nectaries in Aspen (Populus tremuloides): Heritable Genetic Variation and Herbivore-induced Expression
,*
1 Department of Entomology
2 Department of Botany, University of Wisconsin, Madison, WI 53706, USA
* For correspondence. E-mail wooley{at}biology.csustan.edu
Received: 13 June 2007 Returned for revision: 9 July 2007 Accepted: 23 July 2007
Background and Aims: A wide variety of plants produce extrafloral nectaries (EFNs) that are visited by predatory arthropods. But very few studies have investigated the relationship between plant genetic variation and EFNs. The presence of foliar EFNs is highly variable among different aspen (Populus tremuloides) genotypes and the EFNs are visited by parasitic wasps and predatory flies. The aim here was to determine the heritability of EFNs among aspen genotypes and age classes, possible trade-offs between direct and indirect defences, EFN induction following herbivory, and the relationship between EFNs and predatory insects.
Methods: EFN density was quantified among aspen genotypes in Wisconsin on trees of different ages and broad-sense heritability from common garden trees was calculated. EFNs were also quantified in natural aspen stands in Utah. From the common garden trees foliar defensive chemical levels were quantified to evaluate their relationship with EFN density. A defoliation experiment was performed to determine if EFNs can be induced in response to herbivory. Finally, predatory arthropod abundance among aspen trees was quantified to determine the relationship between arthropod abundance and EFNs.
Key Results: Broad-sense heritability for expression (0·74–0·82) and induction (0·85) of EFNs was high. One-year-old trees had 20% greater EFN density than 4-year-old trees and more than 50% greater EFN density than 
10-year-old trees. No trade-offs were found between foliar chemical concentrations and EFN density. Predatory fly abundance varied among aspen genotypes, but predatory arthropod abundance and average EFN density were not related.
Conclusions: Aspen extrafloral nectaries are strongly genetically determined and have the potential to respond rapidly to evolutionary forces. The pattern of EFN expression among different age classes of trees appears to follow predictions of optimal defence theory. The relationship between EFNs and predators likely varies in relation to multiple temporal and environmental factors.
Key words: Aspen, extrafloral nectaries, herbivory, indirect defence, induction, mutualism, optimal defence, Populus tremuloides, heritability, genetic variation
Present address: Department of Biological Sciences, California State University, Stanislaus, Turlock, CA 95382, USA.
Present address: Living Biography, 55 N. University Ave., Ste 223, Provo, UT 84606, USA.