AOBPreview originally published online on January 25, 2008
Annals of Botany 2008 101(4):521-530; doi:10.1093/aob/mcm329
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Are Pectins Involved in Cold Acclimation and De-acclimation of Winter Oil-seed Rape Plants?
ebrowski2,3
1 Department of Plant Molecular Biology, Institute of Experimental Plant Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland
2 Institute of Plant Breeding and Acclimation, Radzików, 95-870 B
onie, Poland
3 Department of Plant Physiology, Faculty of Biotechnology, University of Rzeszow, Rejtana 16c, 35-959 Rzeszów, Poland
* For correspondence. E-mail alka{at}biol.uw.edu.pl
Received: 6 September 2007 Returned for revision: 9 October 2007 Accepted: 4 December 2007 Published electronically: 25 January 2008
Background and Aims: The hypothesis was tested that pectin content and methylation degree participate in regulation of cell wall mechanical properties and in this way may affect tissue growth and freezing resistance over the course of plant cold acclimation and de-acclimation.
Methods: Experiments were carried on the leaves of two double-haploid lines of winter oil-seed rape (Brassica napus subsp. oleifera), differing in winter survival and resistance to blackleg fungus (Leptosphaeria maculans).
Key Results: Plant acclimation in the cold (2 °C) brought about retardation of leaf expansion, concomitant with development of freezing resistance. These effects were associated with the increases in leaf tensile stiffness, cell wall and pectin contents, pectin methylesterase (EC 3·1·1·11) activity and the low-methylated pectin content, independently of the genotype studied. However, the cold-induced modifications in the cell wall properties were more pronounced in the leaves of the more pathogen-resistant genotype. De-acclimation promoted leaf expansion and reversed most of the cold-induced effects, with the exception of pectin methylesterase activity.
Conclusions: The results show that the temperature-dependent modifications in pectin content and their methyl esterification degree correlate with changes in tensile strength of a leaf tissue, and in this way affect leaf expansion ability and its resistance to freezing and to fungus pathogens.
Key words: Brassica napus subsp, oleifera, cell wall, cold acclimation, de-acclimation, freezing, growth, leaf stiffness, pathogen, pectins, pectin methylesterase
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