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AOBPreview originally published online on February 19, 2008
Annals of Botany 2008 101(7):983-995; doi:10.1093/aob/mcn022
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© The Author 2008. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Cellular Ultrastructure and Crystal Development in Amorphophallus (Araceae)

Christina J. Prychid1,*, Rachel Schmidt Jabaily2 and Paula J. Rudall1

1 Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3DS, UK
2 College of Letters and Science, Botany Unit, 256 Birge Hall, 430 Lincoln Drive, University of Wisconsin, Madison, WI 53706, USA

* For correspondence. E-mail c.prychid{at}kew.org

Received: 20 November 2007    Returned for revision: 3 January 2008    Accepted: 22 January 2008    Published electronically: 19 February 2008

Background and Aims: Species of Araceae accumulate calcium oxalate in the form of characteristically grooved needle-shaped raphide crystals and multi-crystal druses. This study focuses on the distribution and development of raphides and druses during leaf growth in ten species of Amorphophallus (Araceae) in order to determine the crystal macropatterns and the underlying ultrastructural features associated with formation of the unusual raphide groove.

Methods: Transmission electron microscopy (TEM), scanning electron microscopy (SEM) and both bright-field and polarized-light microscopy were used to study a range of developmental stages.

Key Results: Raphide crystals are initiated very early in plant development. They are consistently present in most species and have a fairly uniform distribution within mature tissues. Individual raphides may be formed by calcium oxalate deposition within individual crystal chambers in the vacuole of an idioblast. Druse crystals form later in the true leaves, and are absent from some species. Distribution of druses within leaves is more variable. Druses initially develop at leaf tips and then increase basipetally as the leaf ages. Druse development may also be initiated in crystal chambers.

Conclusions: The unusual grooved raphides in Amorphophallus species probably result from an unusual crystal chamber morphology. There are multiple systems of transport and biomineralization of calcium into the vacuole of the idioblast. Differences between raphide and druse idioblasts indicate different levels of cellular regulation. The relatively early development of raphides provides a defensive function in soft, growing tissues, and restricts build-up of dangerously high levels of calcium in tissues that lack the ability to adequately regulate calcium. The later development of druses could be primarily for calcium sequestration.

Key words: Amorphophallus, Araceae, calcium oxalate, crystals, development, druses, raphides, ultrastructure


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