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AOBPreview originally published online on July 28, 2009
Annals of Botany 2009 104(5):975-985; doi:10.1093/aob/mcp184
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© The Author 2009. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages

María Verónica Rodríguez1,*, Guillermina Mónica Mendiondo1, Laura Maskin1, Gustavo Eduardo Gudesblat2,{dagger}, Norberto Daniel Iusem2 and Roberto Luis Benech-Arnold1

1 IFEVA (CONICET-UBA) Facultad de Agronomía, UBA. Av. San Martín 4453 (C1417DSE), Buenos Aires, Argentina
2 LFBM, FCEyN, UBA. Pabellón II, Ciudad Universitaria (1428), Buenos Aires, Argentina

* For correspondence. E-mail mvr{at}agro.uba.ar

Received: 19 March 2009    Returned for revision: 7 May 2009    Accepted: 17 June 2009    Published electronically: 28 July 2009

Background and Aims: Pre-harvest sprouting susceptibility in grain sorghum (Sorghum bicolor) is related to low seed dormancy and reduced embryo sensitivity to inhibition of germination by abscisic acid (ABA). Intra-specific variability for pre-harvest sprouting might involve differential regulation of ABA signalling genes.

Methods: Sorghum genes encoding homologues for ABA signalling components from other species (ABI5, ABI4, VP1, ABI1 and PKABA1) were studied at the transcriptional and protein level (ABI5) during grain imbibition for two sorghum lines with contrasting sprouting phenotypes and in response to hormones.

Key Results: Transcript levels of these genes and protein levels of ABI5 were higher in imbibed immature caryopses of the more dormant line. Dormancy loss was related to lower transcript levels of these genes and lower ABI5 protein levels in both genotypes. Exogenous ABA inhibited germination of isolated embryos but failed to prevent ABI5 rapid decrease supporting a role for the seed coat in regulating ABI5 levels.

Conclusions: Several genes involved in ABA signalling are regulated differently in imbibed caryopses from two sorghum lines with contrasting pre-harvest sprouting response before – but not after – physiological maturity. A role for ABI5 in the expression of dormancy during grain development is discussed.

Key words: Sorghum bicolor, pre-harvest sprouting, abscisic acid, hormone signalling, ABI5, seed dormancy

{dagger} Present address: VIB Department of Plant Systems Biology Ghent University, Technologiepark 927 9052 Gent, Belgium.


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