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AOBPreview originally published online on September 29, 2009
Annals of Botany 2009 104(6):1121-1128; doi:10.1093/aob/mcp215
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© The Author 2009. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Arabidopsis plants harbouring a mutation in AtSUC2, encoding the predominant sucrose/proton symporter necessary for efficient phloem transport, are able to complete their life cycle and produce viable seed

Avinash C. Srivastava{dagger}, Kasturi Dasgupta, Eric Ajieren, Gabriella Costilla, Roisin C. McGarry and Brian G. Ayre*

University of North Texas, Department of Biological Sciences, 1155 Union Circle #305220, Denton TX 76203-5017, USA

* For correspondence. E-mail brian.ayre{at}unt.edu

Received: 18 June 2009    Returned for revision: 21 July 2009    Accepted: 23 July 2009    Published electronically: 29 September 2009

Background and Aims: AtSUC2 encodes a sucrose/proton symporter that localizes throughout the collection and transport phloem and is necessary for efficient transport of sucrose from source to sink tissues in Arabidopsis thaliana. Plants harbouring homozygous AtSUC2 null alleles accumulate sugar, starch, and anthocyanin in mature leaves, have severely delayed development and stunted growth and, in previous studies, failed to complete their life cycle by producing viable seed.

Methods: An AtSUC2 allele with a T-DNA insertion in the second intron was analysed. Full-length transcript from this allele is not produced, and a truncated protein translated from sequences upstream of the insertion site did not catalyse sucrose uptake into yeast, supporting the contention that this is a null allele. Mutant plants were grown in a growth chamber with a diurnal light/dark cycle, and growth patterns recorded.

Key Results: This allele (SALK_038124, designated AtSUC2-4) has the hallmarks of previously described null alleles but, despite compromised carbon partitioning and growth, produces viable seeds. The onset of flowering was chronologically delayed but occurred at the same point in the plastochron index as wild type.

Conclusions: AtSUC2 is important for phloem loading and is therefore fundamental to phloem transport and plant productivity, but plants can complete their life cycle and produce viable seed in its absence. Arabidopsis appears to have mechanisms for mobilizing reduced carbon from the phloem into developing seeds independent of AtSUC2.

Key words: Arabidopsis thaliana, AtSUC2, sucrose, transporter, symporter, phloem, phloem loading, plant productivity

{dagger} Present address: Samuel Roberts Noble Foundation, Plant Biology Division, Ardmore, OK 73401, USA


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