Annals of Botany 35: 159-163, 1971
© 1971 Annals of Botany Company
RESEARCH-ARTICLE |
Effect of External Supply of Growth Substances on Axillary Proliferation and Development in Dioscorea bulbifera
School of Biological Sciences, University of Lagos Lagos, Nigeria
Received: 30 June 1970
Bulbil development in cultured nodes of D. bulbifera proceeded in the absence of growth substances from the medium. When IAA was incorporated into the medium at the concentrations of 5 mg l1 and 10 mg l1 the cultured nodes produced larger bulbils than in its absences. When the concentration of IAA was increased to 15 mg l1, however, the cultured nodes produced a callus instead of a properly organized bulbil. The dry weight of bulbils increased when kinetin was added to the medium at the concentrations of 0.05, 0.5, and 2.5 mg l1. The greatest increase was with 0.5 mg l1 kinetin. On increasing the concentration of kinetin in the medium to 5.0 mg l1 the tissue produced had smaller dry weight than those produced in the absence of growth substances. Addition of different combinations of IAA and kinetin to the basal medium resulted in the production of normal bulbils, roots, and shoots in some instances (suitable combinations) and in the production of callus and abnormal shoots in others (non suitable combinations).