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Annals of Botany 39: 571-577, 1975
© 1975 Annals of Botany Company

RESEARCH-ARTICLE

Regeneration of Diploid and Polyploid Plants from the Stem Pith Explants of Diploid Marrow Stem Kale (Brassica oleracea L.)

J. HORÁK1, J. LUSTINCE, J. MESÍCEK2, M. KAMÍNEK and D. POLÁCKOVÁ3

Institute of Experimental Botany, Czechoslovak Academy of Sciences, Research Station Alsovice, 478 21 Bratrikov, Czechoslovakia
2Institute of Botany, Czechoslovak Academy of Sciences 252 433 Pruhonice
3Institute o Genetics and Plant Breeding 166 00 Praha 6, Ruzyne

1 Present address: Jiráskovo nám. 14, 541 01 Trutnov.

Received: 10 May 1974   

Viable plants of kale (Brassica oleracea L.) have been regenerated from stem pith explants grown on complex agar media. About 80 per cent of kale plants cv. Krasa gave explants which differentiated roots and shoots. Analysis of stomatal length, pollen grain morphology and estimation of chromosome number in PMC and somatic cells showed that a set of 71 regenerated plants derived from five diploid mother plants contained 6 di-, 54 tetra-, and 11 octoploid regenerates. Utilization of this method in plant breeding is discussed.


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