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Annals of Botany 39: 1091-1095, 1975
© 1975 Annals of Botany Company


RESEARCH-ARTICLE

Flavanoids in Pollen and Stigma of Brassica oleracea and Their Effects on Pollen Germination In Vitro

M. SEDGLEY1

Scottish Horticultural Research Institute Invergowrie, Dundee, Scotland

Received: 25 September 1974   

Brassica oleracea pollen was applied to a basic medium of 1.5 per cent agar and 15 per cent sucrose to which flavanoids were added at three concentrations. Two types of agar were used; with agar 1, quercetin at a concentration of 0.5 x 10–3 per cent gave an increase in percentage germinating grains. With agar 2, an increase in germination occurred with kaempferol and naringin at concentrations of 0.5 x 10–3 and 0.5 x 10–1 per cent respectively. Increase in pollen tube length occurred with agar 2 and quercetin at a concentration of 0.5 x 10–3 per cent.

The stigma tissue of B. oleracea contains at least three and the pollen at least one glycoside of quercetin. The sugars in the glycosides were not identified. Pollen germination and pollen tube extension were not stimulated exclusively by the flavanoids present in the stigma. The flavanoid composition of the stigma did not vary amongst five different S-allele genotypes, indicating that flavanoids are probably not directly involved in the incompatibility reaction of B. oleracea.


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