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Annals of Botany 41: 1113-1116, 1977
© 1977 Annals of Botany Company

RESEARCH-ARTICLE

Scanning Electron Microscope Studies of Multiple Shoot Production by Meristem Tip Cultures of Solarium x curtilobum

B. W. W. GROUT, R. J. WESTCOTT and G. G. HENsHAW

Department of Plant Biology, University of Birmingham P.O. Box 363, Birmingham B15 2TT

Received: 25 September 1976   

A meristem tip culture isolated from Solatium x curtilobum cv. Mallku produced a limited amount of callus at the cut surface of the explant when cultured on Murashige and Skoog medium containing 1 mg dm3 6-{gamma}-{gamma}{dimethylallylamino)purine and O.01 mg dm3 naphthalene acetic acid.

A single sheet of cuticle-like material develops on localized regions of the callus surface and the cells beneath it decrease in mean cell size. These meristematic centres develop into fully organized shoot meristems, and each will grow out into a leafy shoot when the culture is transferred to growth medium containing o.1 mg dm3 gibberellic acid (GA3) as the only growth hormone. This system has considerable value in the rapid clonal propogation of Solanum species, since as many as fifty shoots can be produced from a single meristem tip culture.


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