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Annals of Botany 52: 781-789, 1983
© 1983 Annals of Botany Company


RESEARCH-ARTICLE

Detection of Pollen Germination Inhibitors in Brassica oleracea Tissue Extracts

T. HODGKIN and G. D. LYON

Scottish Crop Research Institute Invergowrie, Dundee DD2 5DA, UK

Accepted: 22 March 1983   

Petunia hybrida and Lilium lankongense pollens were germinated on thin layer chromatography (TLC) plates following chromatography of extracts from the self-, cross- and unpollinated stigmas, styles and ovaries and the seeds, leaves and pollen of three inbred Brassica oleracea families. Zones of pollen germination inhibition on the TLC plates showed that inhibitory compounds were present in the tissue extracts. The Rf values and number of these compounds varied with the tissue used, stigma tissue containing the largest amounts and the greatest number of inhibitors. In contrast, differences between the inbred lines tested were slight and quantitative. Pollen from both P. hybrida and L. lankongense gave the same results; that from B. oleracea could not be used because of its poor germination.

Brassica oleracea, Brussels sprout, kale, Lilium lankongense, Petunia hybrida, pollen germination, thin layer chromatography, germination inhibitors, phytoalexin, bioassay


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