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Annals of Botany 52: 891-894, 1983
© 1983 Annals of Botany Company

RESEARCH-ARTICLE

Metabolism of N-(purin-6-yl)glycine by Soya Bean Callus

J. VAN STADEN* and S. E. DREWES{dagger}

* Departments of Botany, University of Natal Pietermaritzburg 3200, Republic of South Africa
{dagger} Departments of Chemistry, University of Natal Pietermaritzburg 3200, Republic of South Africa

Accepted: 14 May 1983   

The growth of cytokinin-dependent soya bean callus has been shown to be accelerated by adding N-(purin-6-yl)glycine to the medium. Two biologically active peaks were detected when the callus was cultured with N-(purin-6-yl)glycine. These two peaks co-chromatographed with 6-(2, 3, 4-trihydroxy-3-methylbutylamino)purine and zeatin respectively. When 14C labelled N-(purin-6-yl)glycine was applied to the callus, radioactivity was found with both these compounds irrespective of whether or not the N-(purin-6-yl)glycine was labelled in the side chain or in the 8-position of the purine ring. Small amounts of zeatin appear to be produced from N-(purin-6-yl)glycine which could explain why this compound stimulates the division of soya bean callus.

N-(purin-6-yl)glycine, soya bean callus, metabolism, radioactivity, cytokinins


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