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Annals of Botany 61: 373-375, 1988
© 1988 Annals of Botany Company

RESEARCH-ARTICLE

Effect of Spermidine and Methylglyoxal-bis (Guanyl-hydrazone) (MGBG) on In Vitro Pollen Germination and Tube Growth in Catharanthus roseus

L. PRAKASH, PHILIP JOHN, G. M. NAIR and G. PRATHAPASENAN

Department of Botany, The Maharaja Sayajirao University of Baroda Baroda-390002, India

Accepted: 13 November 1987   

Incorporation of polyamine-spermidine into the nutrient medium at 10–6 and 10–5 M concentrations stimulates pollen-tube growth in vitro in Catharanthus roseus L. G. Don. MGBG, an inhibitor of spermidine biosynthesis, at 0.5 x 10–3 and 1 x 10–3 M concentrations reduced the percentage of germination as well as tube growth and at a concentration of 1.5 x 10–3 M germination was totally inhibited. Pollen grains incubated in the medium containing 1.5 x 10–3 M MGBG, when transferred to a fresh medium with 10–5 M spermidine, resulted in 80% germination recovery, along with considerable tube growth. Experiments with actinomycin-D indicate that stimulation of pollen-tube growth by spermidine may involve de novo synthesis of protein.

Catharanthus roseus, pollen germination, tube growth, spermidine, MGBG, inhibition, actinomycin-D


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