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Annals of Botany 61: 393-394, 1988
© 1988 Annals of Botany Company

RESEARCH-ARTICLE

Preservation of the Differentiated State of Mesophyll Cells In Vitro

A. ASKANI and R. BEIDERBECK

Botanisches Institut der Universitat Heidelberg Im Neuenheimer Feld 360, 6900 Heidelberg, FRG

Accepted: 5 December 1987   

Under a variety of light conditions, and in a modified NT medium containing sucrose as a carbon source, isolated mesophyll cells of Calystegia sepium started cell divisions after 72 h. Alternately, under light-dark alterations (12: 12 h; 1.35–1.50 W m–2 of cool white light), and culture in the NT medium lacking an organic carbon source, and made iso-osmotic by the addition of mannitol, the cells preserved their differentiated state as regards cell size, cell form, colour and size and distribution of chloroplasts for one week. Later on, they changed their character and cell division was started.

Calystegia sepium, mesophyll cells, differentiated state, in vitro culture


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