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Annals of Botany 62: 481-486, 1988
© 1988 Annals of Botany Company

RESEARCH-ARTICLE

The Theory of Diffusion of Solutes in Pollen; Theory, Design and Experimental Procedure

M. L. SOOD

Department of Chemistry, Punjab Agricultural University Ludhiana-141004, India

Accepted: 18 May 1988   

The theory of diffusion of solutes in pollen is developed and used to derive formulae from which the mean diffusion coefficient () can be determined. A mathematical relation ßPGC t = ln ({delta}C0/{delta}Cf) is developed by employing Fick's law. In this relation is the average diffusion coefficient, ßPGC is a constant characteristic of the pollen, the ‘Pollen Grain Constant’, {delta}C0 and {delta}Cf are the initial and final concentration differences between the solution of the solute and the pollen grain and t is the time of diffusion in seconds. A diffusion apparatus has been designed and made. The design is simple to operate and an experimental procedure is described to determine the ßPGC using dilute potassium chloride solutions. Knowing the pollen grain constant, the method to determine diffusion coefficient () with any other solute is explained.

Diffusion, design, pollen, solutes, theory


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