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Annals of Botany 62: 513-519, 1988
© 1988 Annals of Botany Company

RESEARCH-ARTICLE

Development of a Superficial Meristem During Somatic Embryogenesis from Immature Cotyledons of Soybean (Glycine max L.)

A. HEPHER*, M. E. BOULTER{dagger}, N. HARRIS{dagger} and R. S. NELSON*

*Shell Research Ltd, Sittingbourne Research Centre Sittingbourne, Kent, ME9 8AG, UK*
{dagger}Department of Botany, University of Durham, Science Laboratories South Road, Durham, DH1 3LE, UK{dagger}

Accepted: 2 June 1988   

Initial events were studied in the development of an embryogenic meristem during somatic embryogenesis from in vitro cultured immature cotyledons of soybean. The presence of 2,4-D in the culture medium led to the formation of a superficial embryogenic tissue associated with the abaxial epidermis of 3 mm cotyledons. Additionally, 2,4-D initiated rapid non-morphogenic periclinal division in the parenchyma tissues of the cotyledon. Consequent internal expansion disrupted and eventually ruptured the apparently quiescent adaxial epidermis. The profound difference in the in vitro response between abaxial and adaxial epidermes is discussed in relation to their relative roles in nutrient transport during seed development in vivo.

Somatic embryogenesis, transfer cells, Glycine max


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