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Annals of Botany 64: 625-633, 1989
© 1989 Annals of Botany Company

RESEARCH-ARTICLE

Cell Cycle Changes in the Shoot Apex of Silene coeli-rosa During the Second and Third Days of Floral Induction

M. TAYLOR and D. FRANCIS

School of Pure and Applied Biology, University of Wales College of Cardiff P.O. Box 915, Cardiff CFI 3TL, UK

Accepted: 12 May 1989   

The cell cycle in Silene coeli-rosa shoot apices was measured to test whether or not early components of the floral stimulus, produced during the 2nd and 3rd long days (LD) of an inductive LD treatment, resulted in an increase in the duration of G2 phase in constant 20–24 h cell cycles. Plants were grown at 20°C in short days (SD) of 8 h light and 16 h darkness for 28 d (day 0). Starting on day 0, plants were given SD or 3 LD each comprising an identical 8 h day and 16 h photo-extension, or 3 dark-interrupted (d.i.) non-inductive LD, interrupted at 1700 h of each day with 1 h of darkness. The cell cycle (percentage labelled mitoses method) and changes in cell number were determined in the shoot apical meristem. During days 1–2 of the SD treatment, the cell cycle and mean cell generation time (MCGT) was 18 and 32 h, respectively, giving a growth fraction of 56%. During days 2–3, the cell cycle and MCGT shortened to 15 and 23 h, respectively (growth fraction = 65%). During days 1–2 of the LD and d.i. LD treatments, cell cycles and MCGTs were 9–10 and 27–29 h, respectively, resulting in smaller growth fractions (about 33%). Thus, shortened cell cycles and altered growth fractions occurred regardless of whether or not the treatment was inductive. The LD treatment resulted in a marked shortening of G1 and, to a lesser extent, S-phase, whilst G2 remained constant. These changes were consistent with increases in the proportion of cells in G2 during the photoextension of each LD which were suppressed during the comparable periods of the d.i. LD treatment. The latter treatment resulted in each phase occupying virtually identical proportions of the cell cycle as in the SD treatment. Thus, the unique cell cycle responses to the initial part of the inductive LD treatment were increases in the proportion of cells in G2 coupled with G1 and G2 being of similar duration.

Cell cycle, mean cell generation time, shoot apex, Silene coeli-rosa


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