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Annals of Botany 72: 239-247, 1993
© 1993 Annals of Botany Company

Comparison of Plant Telomere Locations using a PCR-generated Synthetic Probe

Antony V. Cox, Simon T. Bennett, Alexander S. Parokonny, Ann Kenton, Markella A. Callimassia and Michael D. Bennett

Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3DS, UK

We have generated a telomere-specific probe by the polymerase chain reaction and used it to localize chromosome telomeres of ten unrelated angiosperm species in in situ. Concatenation of the simple monomers, 5'-(TTTAGGG)-3', derived from the sequence of Arabidopsis thaliana telomeres, yielded a stable, versatile and reliable probe that gave a signal of high intensity following fluorescence in situ hybridization. Most species, including those with known karyotype rearrangements, showed telomere label only at chromosome termini. These findings are discussed in the context of the chromosomal events responsible for generating and stabilizing karyotype change in plants.Copyright 1993, 1999 Academic Press

PCR, telomere repeat sequence (TRS), in situ hybridization, comparative physical mapping, karyotype evolution, genome organization, Lathyrus sativus L., Vicia sativa L. subsp. nigra (L.) Ehrh., Vigna radiata (L.) R. Wilczek cv. Berken, Nicotiana sylvestris Speg et Comes, Haplopappus gracilis (Nutt.) A. Gray, Gibasis pulchella (Kunth) Rafin., Tradescantia commelinoides Schultes. fil., Hordeum vulgare L. cv. Sultan., Hordeum vulgare L. cv. Tuleen 346, Milium vernale Bieb., Paphiopedilum insigne (Wall.) Pfitz


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