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Annals of Botany 74: 53-58, 1994
© 1994 Annals of Botany Company

Alternating Cytokinins in Multiplication Media Stimulates in Vitro Shoot Growth and Rooting of Eucalyptus globulus Labill

I. J. Bennett*,{ddagger}, J. A. McComb*, C. M. Tonkin{dagger} and D. A. J. McDavid*

* School of Biological and Environmental Sciences, Murdoch University Murdoch, Western Australia, 6150
{dagger} DLO-Research Institute for Agrobiology and Soil Fertility (AB-DLO) P.O. Box 14, 6700 A A Wageningen, The Netherlands

{ddagger} For correspondence at: Department of Applied Science, Edith Cowan University, Mount Lawley, Western Australia, 6050, Australia.

Received: 25 February 1993    Accepted: 15 January 1994   

Shoots of Eucalyptus globulus Labill. cultured on shoot multiplication media containing, on alternate subcultures, 6-benzylaminopurine (BAP) or 6-furfurylaminopurine (kinetin), showed better growth than cultures in which either of the cytokinins was used continuously, or both were used in an equimolar mixture. When BAP was used continuously in the medium (i.e. in every subculture), shoots multiplied but remained stunted and leaves became red and abscised. Kinetin or 6-dimethylallyaminopurine (2iP) used continuously in the medium induced very low multiplication but the shoots did not become red nor did the leaves abscise. Shoots taken from multiplication medium containing BAP and placed on rooting medium with 10 µM indole butyric acid (IBA) produced few roots and often died while on the rooting medium. In contrast, shoots from the multiplication medium containing kinetin produced more roots and remained healthy during the passage on the rooting medium.

Eucalyptus globulus, Tasmanian bluegum, cytokinins, micropropagation, in vitro rooting, simulation model, source-sink


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