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Annals of Botany 74: 417-422, 1994
© 1994 Annals of Botany Company

Phosphoenolpyruvate Carboxylase from Heterotrophically Cultured Catharanthus roseus Cells

Mayumi Nagano, Akiko Hachiya and Hiroshi Ashihara

Department of Biology, Faculty of Science, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112, Japan

Maximum activity of phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) was detected at the stationary phase of growth of Catharanthus roseus cells in a heterotrophic culture. The activity of PEPC, after partial purification by fractionation with ammonium sulphate and chromatography on Q-Sepharose, was greatly influenced by pH. The Km of phosphoenolpyruvate (PEP) was 23 µM at pH 8·0 and 45 µM at pH 7·4. Malate, aspartate, citrate, ATP, pyrophosphate and Pi acted as inhibitors of PEPC, but the extent of inhibition varied in each case with the pH of the reaction mixture. By contrast, glucose-6-phosphate, fructose-1,6-bisphosphate and acetyl-CoA, known as stimulators of the activity of PEPC from other sources, had little or no effect on the activity of the partially purified PEPC. The possible role and mechanism of regulation of PEPC in C. roseus cells are discussed.Copyright 1994, 1999 Academic Press

Catharanthus roseus, Apocynaceae, Madagascar periwinkle, suspension culture, phosphoenolpyruvate carboxylase, enzyme kinetics, glycolysis


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