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Annals of Botany 74: 541-546, 1994
© 1994 Annals of Botany Company

Cryopreservation for Seedbanking of Australian Species

D. H. Touchell and K. W. Dixon

Kings Park and Botanic Garden, West Perth, Western Australia, 6005, Australia

Recent studies have indicated that a large number of native Australian species can be stored in liquid nitrogen using relatively straightforward methods. Furthermore, it is estimated that seed from at least 40% of the rare and endangered species of Western Australia could be maintained through use of these cryopreservation techniques. However, before cryopreservation of seed of Australian species can be implemented as a routine technique, physical and chemical properties of seed must be studied. This study has shown that successful cryopreservation of seed could be influenced singly or by a combination of hardseededness, moisture, lipid content, fatty acid and amino acid composition. The study also details the role of in vitro methods for determining seed viability/recovery of species that are either damaged by liquid nitrogen or are difficult to germinate.Copyright 1994, 1999 Academic Press

Seed, cryopreservation, lipid, fatty acid, amino acid, Australian species


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