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Annals of Botany 75: 267-275, 1995
© 1995 Annals of Botany Company

The Cytokinin Preference for in vitro Germination and Protocorm Growth of Cypripedium candidum

M. A. De Pauw, W. R. Remphrey and C. E. Palmer

Department of Plant Science, University of Manitoba, Winnipeg, Manitoba, R3T 2N2 Canada

Cytokinin preference and optimum levels were investigated for in vitro germination and protocorm growth of Cypripedium candidum Muhl. ex Willd. (Lady's Slipper Orchid) using seed collected 8 weeks after pollination. Benzyl adenine (BA) and 6-({alpha},{alpha}-dimethylallylamino)-purine(2iP) in concentrations of up 0·8 mg 1-1 enhanced germination significantly in comparison to controls without cytokinin. Kinetin did not enhance germination. The pattern of germination over time in culture varied with cytokinin type. After 4 and 8 weeks, a higher germination percentage occurred on media with BA and 2iP. Nine morphological types of protocorms were identified and a model to explain protocorm development is proposed. Protocorms developed faster and the production of multiple protocorms increased with all three cytokinins in comparison to the control. However, the type of protocorms observed on the kinetin and control treatments was different from that observed an BA and 2iP and may have resulted via a different pathway. BA at the highest concentrations delayed root development.Copyright 1995, 1999 Academic Press

Asymbiotic, germination, Cypripedium candidum, lady's slipper, cytokinin, protocorm development


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