Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Christou, P.
Right arrow Articles by Ford, T.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Christou, P.
Right arrow Articles by Ford, T.
Agricola
Right arrow Articles by Christou, P.
Right arrow Articles by Ford, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 75: 407-413, 1995
© 1995 Annals of Botany Company

Parameters Influencing Stable Transformation of Rice Immature Embryos and Recovery of Transgenic Plants using Electric Discharge Particle Acceleration

Paul Christou and Tameria Ford

Agracetus, 8520 University Green, Middleton, WI 53562, USA

Recovery of transgenic rice plants from elite varieties was reported from the author's laboratory. By using electric discharge particle acceleration, recombinant technology was extended to commercially important japonica and indica rice cultivars not amenable to conventional transformation methodologies. Critical parameters influencing the recovery of transformed embryogenic callus from which transgenic plants were recovered have been defined. Such parameters included the physiological condition of explants prior to bombardment, DNA and gold particle loading rates, accelerating voltage, and depth of particle penetration. Selection for recovery of stable transformants was not critical in this transformation/regeneration system; both selected and non-selected tissues yielded transformed embryogenic callus and plants at approximately similar frequencies.Copyright 1995, 1999 Academic Press

Oryza sativa L., transformation parameters, transgenic indica and japonica plants, particle bombardment, ß-glucuronidase


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.