Annals of Botany 75: 449-454, 1995
© 1995 Annals of Botany Company
Recovery of Chimeric Rice Plants from Dry Seed using Electric Discharge Particle Acceleration
Agracetus Inc., 8520 University Green, Middleton, WI 53562, USA
The development of an efficient system for the creation of transgenic rice plants from immature embryo tissue was described previously. That system resulted in the generation of clonal plants, probably derived from a single cell or a very small number of cells. Creation of chimeric cereal plants is of interest in studies directed towards the elucidation of developmental pathways. We were able to take advantage of the relative ease with which rice axillary buds, in a germinating seedling, can from embryogenic callus or be induced to form multiple tillers. Particle bombardment of such tissues, at various stages of development, resulted in the recovery of transgenic plants that were chimeric. The creation, origin and development of chimeric cereal plants can thus be studied and new insight into fundamental aspects of early plant development can be obtained. This regeneration/transformation system is compared with a previously developed protocol involving gene transfer into immature embryos which resulted in the exclusive recovery of clonal plants. It is also compared and contrasted with a system described earlier for soybean engineering that resulted on the recovery both chimeric and clonal plants.Copyright 1995, 1999 Academic Press
Oryza sativa, transformation, chimeric plant phenotypes, development, ß-glucuronidase, particle bombardment