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Annals of Botany 75: 463-467, 1995
© 1995 Annals of Botany Company

ß-Amylase Activity as an Index for Germination Potential in Rice

Somen Nandi, Gitali Das and Swati Sen-Mandi

Seed Biology Laboratory, Bose Institute, Calcutta-700 009, India

Seeds of different vigour also differ in their germination ability. In rice (Oryza sativa), this difference was correlated with the level of incorporation of 35S-methionine into 25-60% ammonium sulphate precipitable material that was rich in amylase proteins. This protein fraction, from dry seeds, contained no {alpha}-amylase activity. In contrast, ß-amylase activity was present in all seed stocks capable of 99% germination, although the level was lower in seeds that grew slowly when germinated. In low viability low vigour stock (i.e. extensively deteriorated seeds) ß-amylase activity was absent. Alpha-amylase activity in all stocks was detected only after 24 h from the start of imbibition. These results indicate that ß-amylase activity is reliable indicator of the germination ability of rice seed stocks and of their vigour during germination.Copyright 1995, 1999 Academic Press

Rice (Oryza sativa L.,), germination, ß-amylase, {alpha}-amylase, seed vigour


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