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Annals of Botany 76: 331-335, 1995
© 1995 Annals of Botany Company

Content and Distribution of Erythroxylum coca Leaf Alkaloids

Emanuel L. Johnson

United States Department of Agriculture, Agricultural Research Service, Plant Sciences Institute, Weed Science Laboratory, BARC-W, Beltsville, MD 20705-2350 USA

Fully expanded leaves of Erythroxylum coca var. coca Lam. (Erythroxylaceae) 35-70-d-old were harvested from 21-month-old plants grown under greenhouse conditions. Each harvested leaf was placed on a plexiglass silhouette according to its dimensions and divided into four primary sections (petiole, base, mid and anterior) and three subsections (lamina periphery, false mid-rib, and true mid-rib) to determine the distribution and content of hygrine, cuscohygrine, trans-cinnamoylcocaine, cis -cinnamoylcocaine, tropacocaine, tropinone, methyl ecgonine and cocaine. The leaf sub-sections and petiole were extracted and analysed for alkaloid content (%) by HPLC (cocaine alkaloid only) gas chromatography and GC/MS. Cocaine, methyl ecgonine and hygrine were highest in the lamina periphery with a content of 0·48, 0·46 and 0·32%, respectively. Trans-cinnamoylcocaine was pre-eminent of the cinnamoylcocaines and was most abundant in the petiole at a content of 0·24%. Cis-cinnamoylcocaine, tropinone, cuscohygrine and tropacocaine were ubiquitously distributed throughout the leaf where the average contents were 0·14, 0·004, 0·16 and 0·04%, respectively.Copyright 1995, 1999 Academic Press

Alkaloids, Erythroxylum coca var. coca, E. coca, leaf alkaloids, petiole alkaloids, cocaine, cuscohygrine, methyl ecgonine, hygrine, tropinone, tropacocaine, trans-cinnamoylcocaine, cis-cinnamoylcocaine


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