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Annals of Botany 77: 413-420, 1996
© 1996 Annals of Botany Company

The Structure and Fungal Associates of Mycorrhizas in Leucopogon parviflorus (Andr.) Lindl.

E. STEINKE, P. G. WILLIAMS and A. E. ASHFORD

School of Biological Science, The University of New South Wales, Sydney, NSW 2052, Australia

March 15, 1995 ; August 28, 1995

Mycorrhizal root systems ofLeucopogon parviflorus (Andr.) Lindl. were collected from wild populations at three sites on the coast of New South Wales, Australia and examined by light and electron microscopy. The structure of the hair roots is typical of the family, there being an epidermal layer in which ericoid mycorrhizas are formed, two cortical layers (an exodermis and endodermis) and a very small stele. The colour, size and coil structure of the fungal symbionts indicate that there were at least two different fungi that consistently formed ericoid mycorrhizal structures at these sites. Transmission electron microscopy of the endophytes showed only ascomycete fungi. Plants from two of the populations were used for fungal isolations. Fungi were isolated by incubating surface sterilized hair-root pieces in a solution of bovine serum albumin with penicillin and streptomycin. Twenty-one different culture types were obtained, four of which were common to both sites. Two of the common culture types were dark, sterile, slow-growing cultures similar to the ericoid endophyteHymenoscyphus ericae(Read) Korf & Kernan.

Leucopogon parviflorus ; bearded heath; Epacridaceae; Ericaceae; mycorrhiza; ultrastructure; endophytes; callose


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