Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by GUERRA, M.
Right arrow Articles by BENNETT, M. D.
Right arrow Search for Related Content
PubMed
Right arrow Articles by GUERRA, M.
Right arrow Articles by BENNETT, M. D.
Agricola
Right arrow Articles by GUERRA, M.
Right arrow Articles by BENNETT, M. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 78: 157-161, 1996
© 1996 Annals of Botany Company

rDNA Sites in Mitotic and Polytene Chromosomes of Vigna unguiculata (L.) Walp. and Phaseolus coccineus L. Revealed by in situ Hybridization

M. GUERRA+, A. KENTON and M. D. BENNETT

Departamento de Genética, CCB, Universidade Federal de Pernambuco, 50.732-970, Recife, PE, Brazil Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AB, U.K.

May 4, 1995 ; January 22, 1996

Root tip mitotic and tapetal polytene cells ofVigna unguiculata andPhaseolus coccineus were hybridized with a ribosomal DNA (rDNA) probe. While the number of rDNA sites were as expected forP. coccineus, it was surprisingly higher inV. unguiculata where ten rDNA sites were found in both tissues. A sequential banding technique on mitotic chromosomes ofV. unguiculata was used to map the positions of the rDNA sites more accurately. In mitotic cells eight of the rDNA hybridization sites were similar in size while the remaining sites were smaller. In contrast, the hybridization sites were more variable in size in polytene cells with no more than six sites being relatively large. The differences in size of the hybridization sites between the two tissues suggest differential amplification of the rDNA sequences. InP. coccineus six hybridization sites were found in both tissue types. The relative sizes of the sites were similar in both tissue. The presence of speckled signal surrounding four of the six sites suggested that at least four of the rDNA sites were transcribed.

rDNA; in situ ; Vigna ; Phaseolus ; polytene; tapetal; Leguminosae


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
A. B. MARCON, I. C. L. BARROS, and M. GUERRA
Variation in Chromosome Numbers, CMA Bands and 45S rDNA Sites in Species of Selaginella (Pteridophyta)
Ann. Bot., January 2, 2005; 95(2): 271 - 276.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.