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Annals of Botany 78: 245-253, 1996
© 1996 Annals of Botany Company

Cell Cycle Activation During Imbibition and Visible Germination in Embryos and Megagametophytes of Jack Pine (Pinus banksiana Lamb.)

JANET WYMAN, M-FRANCINE TREMBLAY and SYLVIE LALIBERTÉ

Groupe de Recherche en Ecologie Forestière, Département des sciences biologiques, Université du Québec à Montréal, Case postale 8888, succursale Centre-Ville, Montréal (Québec), Canada, H3C 3P8 Département des sciences appliquées, Unité de recherche et de développement forestier de l'Abitibi-Témiscamingue, Université du Québec en Abitibi-Témiscamingue, Case Postale 700, Rouyn-Noranda (Québec), Canada, J9X 5E4

October 23, 1995 ; March 6, 1996

Flow cytometric determination of cell cycle activation during imbibition and visible germination in five families of jack pine (Pinus banksiana Lamb.) embryos and megagametophytes revealed that in seeds that had undergone no imbibition the majority of cells were in the 2C state. As the imbibition period increased, less of the nuclei were blocked in the G0/G1 state and more become active in the cell cycle. The augmentation in the nuclei active in the 2C–4C cycle as well as those with DNA levels higher than the 4C state occured gradually and preceeded radicle emergence. In megagametophyte tissue examined at various stages of imbibition, cell cycle activity became apparent rapidly following imbibition. In nuclei of green and white embryos examined separately the {chi}2frequency distributions were significantly different for all three families after 144h. As imbibition period increased, fewer nuclei from the green embryos were blocked in the 2C state, and more became active in the 2C–4C cell cycle. This was not the case for white embryos where no significant linear relation was noted. Cell cycle activity in the hypocotyl+cotyledons region and the emerging radicle were examined separately. Functional relations found in the hypocotyl+cotyledons region were not evident in the radicle. As visible germination proceeded, cell cycle activity in the hypocotyl + cotyledons region for this period of germination showed a reversal of the activity noted during imbibition: fewer nuclei were active and once again a higher proportion were found in the 2C state.

cell cycle; C levels; DNA content; flow cytometry; germination; imbibition; jack pine; megagametophyte; Pinus banksiana Lamb


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