Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by OHSUGI, R.
Right arrow Articles by MURATA, T.
Right arrow Search for Related Content
PubMed
Right arrow Articles by OHSUGI, R.
Right arrow Articles by MURATA, T.
Agricola
Right arrow Articles by OHSUGI, R.
Right arrow Articles by MURATA, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 79: 179-184, 1997
© 1997 Annals of Botany Company

Leaf Anatomy and Carbon Discrimination in NAD-malic Enzyme Panicum Species and their Hybrids Differing in Bundle Sheath Cell Ultrastructure

RYU OHSUGI+, OSAMU UENO, TOSHINORI KOMATSU, HARUTO SASAKI and TAKAO MURATA

Department of Applied Physiology, National Institute of Agrobiological Resources, Tsukuba, Ibaraki, Japan, 305 Department of Breeding, National Grassland Research Institute, Nishinasuno, Tochigi, Japan, 329–27 Faculty of Agriculture, The University of Tokyo, Tokyo, Japan, 113 Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan, 020

December 12, 1995 ; August 28, 1996

The relationship between leaf anatomy, ultrastructure and carbon discrimination was investigated in leaves of two F1hybrids (F1-1 and F1-2) between two different types of the grassPanicum [an NAD-malic enzyme (ME) C4species], which differ in bundle sheath ultrastructure. The female parent was Kabulabula grass, which has centrifugal chloroplasts in bundle sheath cells and is designated an NAD-ME(F) species, while the male parent was Makarikari grass, which has centripetal chloroplasts in the bundle sheath cells and is designated an NAD-ME(P) species. Suberin lamellae are present in Kabulabula grass but are lacking in Makarikari grass. Both F1hybrids had the same chromosome number (2n =36) as the parents but exhibited both univalent (about 45%) and bivalent (about 55%) chromosome pairing which was the major basis for the identification of F1hybrids. In F1-1, elongated bundle sheath cell chloroplasts are arranged mainly in a centripetal position, similar to those in the male parent, Makarikari grass. In contrast, most of the bundle sheath cells in F1-2 are packed with starch-containing chloroplasts, although in some cells chloroplasts tended to be centripetally arranged. In both F1hybrids, suberin lamellae were found in the bundle sheath cell walls, similar to the female parent, Kabulabula grass. The {delta}13C values of both F1hybrids were -11.4 to -11.7{per thousand}, almost the same as those of Kabulabula grass (-11.4{per thousand}), but significantly higher than those of Makarikari grass (-12.7{per thousand}). These results indicate that the chloroplast orientation in the bundle sheath cells and the presence of suberin lamellae are not obligatorily linked in their expression and suggest that suberin lamellae may play an important role in discrimination against13C.

Panicum ; NAD-malic enzyme species; hybrid; chloroplast position; 13C discrimination; suberin lamellae


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
H. Kobayashi, M. Yamada, M. Taniguchi, M. Kawasaki, T. Sugiyama, and H. Miyake
Differential Positioning of C4 Mesophyll and Bundle Sheath Chloroplasts: Recovery of Chloroplast Positioning Requires the Actomyosin System
Plant Cell Physiol., January 1, 2009; 50(1): 129 - 140.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.