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Annals of Botany 79: 593-600, 1997
© 1997 Annals of Botany Company

Water Deficit in Pea Root Tips: Effects on the Cell Cycle and on the Production of Dehydrin-like Proteins

MARCELLA BRACALE, MARISA LEVI, CHRISTIAN SAVINI, WALTER DICORATO and MARIA GRAZIA GALLI

Universita' degli Studi di Milano, C.N.R. and Dipartimento di Biologia, Sezione di Botanica Generale, via Celoria 26, 20133, Milano, Italy Universita' di Verona, Istituto Policattedra della Facolta' di Scienze, Strada Le Grazie, Ca' Vignal 1, 37134, Verona, Italy

Received July 12, 1996 ; Accepted October 21, 1996

Dehydrin-like proteins have been detected in nuclei and cytoplasm of meristematic root tip cells from pea seedlings subjected to slow dehydration at 90% relative humidity for 48 h or more. Evidence was gained from Western blotting and immunocytochemical experiments using an antibody raised against the conserved domain of dehydrin proteins. Flow cytometer analysis has shown that cycling cells of root tip meristems from dehydrated seedlings are mostly arrested in G2 phase. Other stress treatments thought to involve water depletion (osmotic stress, cold treatment) or to modulate cell response to water deficit (abscisic acid) gave less clear-cut results with all treatments lowering the proportion of cells entering the S phase, but without a definite and persistent arrest in any preferential phase of the cycle. Possible interrelationships between G2 arrest and dehydrin production are discussed.

Cell cycle; dehydrins; flow cytometry; nuclei; pea; Pisum sativum L.; water stress


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