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Annals of Botany 80: 139-145, 1997
© 1997 Annals of Botany Company

Ultrastructure and Radiolabelling of Leaf Cuticles from Ivy (Hedera helixL.) Plantsin vitroand duringex vitroAcclimatization

C. GILLY, R. ROHR and A. CHAMEL+,

Laboratoire Transferts dans les Systèmes Végétaux, DBMS, CEA/Grenoble, 17 rue des Martyrs, 38054, Grenoble, Cedex 9, France Interactions et Micropropagation, bât. 405, Université Claude Bernard, Lyon 1, 69622, Villeurbanne, Cedex, France

Received August 13, 1996 ; Accepted February 17, 1997 .

Cuticle ultrastructure and radiolabelling of isolated cuticles after incorporation of [14C] acetate in foliar discs were investigated with ivy plants grownin vitrothenex vitro. Results show an increase in thickness, mass and wax content, between young and expanded leaves, for bothin vitroandex vitrocuticles. The cuticle ofin vitrounexpanded leaves was very thin and only constituted a lamellate zone. The ultrastructure ofin vitroyoung and expanded leaf cuticles showed characteristics similar toin situcuticles. The thickness of the lamellate zone remained fairly constant and represented 33% of the cuticle thickness in young leaves, but only 11.4% in expanded leaves. The number of lamellar units decreased from 14 to nine between these two growth stages. The main difference between young leaves developedin vitroorex vitrowas a thinner lamellate zone forex vitrocuticles. However, these latter cuticles had an intermediary zone between the lamellate and reticulate zones. The cuticle thickness of expanded leaves was greater forin vitrocuticles suggesting a temporary decrease in cuticle biosynthesis after transfer of the plant fromin vitrotoex vitro.Results from cuticle radiolabelling show higher radioactivity incorporation in cuticles isolated from leaves developedex vitrocompared toin vitro. This radiolabelling was particularly marked forex vitroyoung leaf cuticles and depended on the duration of theex vitrogrowth period revealing a progressive activation of cuticle biosynthesis in response to new environmental conditions.

Hedera helix; ivy leaf cuticle; in vitroplants; electron microscopy; radiolabelling; isolated cuticles


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