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Annals of Botany 80: 327-333, 1997
© 1997 Annals of Botany Company

The Distribution and Organization of Ty1-copia-like Retrotransposable Elements in the Genome ofVigna unguiculata(L.) Walp. (Cowpea) and its Relatives

I. GALASSO+,,, G. E. HARRISON, D. PIGNONE, A. BRANDES and J. S. HESLOP-HARRISON

Istituto del Germoplasma, C.N.R., via G. Amendola 165/A, 70126, Bari, Italy Karyobiology Group, Department of Cell Biology, John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK

March 3, 1997 ; April 18, 1997 .

Ty1-copia-like retrotransposable elements were amplified fromVignaspecies by the polymerase chain reaction (PCR) with primers derived from the conserved region of the reverse transcriptase (RT) gene. Southern blots of genomic DNA digests fromVigna unguiculataand related species probed with the PCR derived sequences fromV. unguiculata(rt-cow),V. unguiculatasubsp.dekindtiana(rt-dek),V. luteola(rt-lut) andV. vexillata(rt-vex) showed variable hybridization patterns and intensities, generally correlating with taxonomic relationships. Sequencing of four clones revealed homologies to the reverse transcriptase gene of known Ty1-copia-like elements, although comparison of the predicted peptide sequences with each other and previously isolated reverse transcriptase genes from other legumes typically showed less than 50% identity. Fluorescencein situhybridization of the PCR products showed that Ty1-copiaelements represented a major fraction of the genome and were dispersed relatively uniformly over all chromosomes, with exclusion from centromeric, subtelomeric and nucleolar organizing regions, and a few sites of greater abundance. We are now able to start to understand the organization, variability and evolution of retrotransposons within theFabaceae.

Vigna unguiculata; cowpea; retrotransposons; genome organization; in situhybridization; molecular evolution


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