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Annals of Botany 80: 561-564, 1997
© 1997 Annals of Botany Company

A Seed Treatment for Eliminating Non-hybrid Plants when using Atrazine Resistance as a Genetic Marker for Hybrid Seed Production

A. J. CONNER and M. C. CHRISTEY

New Zealand Institute for Crop & Food Research Ltd, Private Bag 4704, Christchurch, New Zealand

Received December 23, 1996 ; Accepted May 12, 1977 .

Brassica plants, with a combination of chloroplasts encoding triazine resistance and mitochondria encoding cytoplasmic male sterility, offer new opportunities for hybrid seed production. Such plants can be grown as a random mixture with a male parent, thereby allowing more effective pollen transfer for greater efficiency of hybrid seed production. Harvested seed consists of hybrid seed from the ‘female’ parent, which is triazine-resistant, and non-hybrid, triazine-sensitive seed resulting from self pollination of the ‘male’ parent.

This study demonstrates that triazine-sensitive broccoli (Brassica oleraceaL. var.italica) progeny can easily be eliminated by allowing the seed to imbibe a solution of 10 g l-1atrazine overnight then drying-back the seed for subsequent germination. Such a treatment prevents triazine-sensitive broccoli from developing beyond the cotyledon stage following germination, and remains effective for at least 3.5 years after seed treatment. The growth of triazine-resistant broccoli plants is unaffected by this seed treatment.

Atrazine; Brassica oleracea; broccoli; hybrid seed; triazine resistance; seed treatment


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