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Annals of Botany 80: 741-746, 1997
© 1997 Annals of Botany Company

Isozyme and RAPD Analysis of the Genetic Diversity Within and BetweenVigna luteolaandV. marina

GABRIELLA SONNANTE+,, ANNE SPINOSA, ANGELA MARANGI and DOMENICO PIGNONE

C.N.R. Istituto del Germoplasma, Via Amendola 165/A, 70126, Bari, Italy

June 2, 1997 ; August 4, 1997 .

Nineteen accessions ofVigna luteola,five ofV. marinassp.oblonga, and two ofV. marinassp.marinawere analysed using variation of isozymes and RAPD markers to obtain better insight into genetic relationships within and between these taxonomic entities. Thirteen putative isozyme loci were scored, seven of which were polymorphic. Both species showed very low genetic diversity indices and most of the variation was detected among populations. Pairwise Nei's genetic distances based on allozyme frequencies were also very low and the accessions ofV. marinassp.marinawere the least related to the others. RAPD analysis was more discriminating and 66 bands out of a total of 85 were polymorphic. Based on the presence or absence of bands, Jaccard's similarity index was calculated. Similarity ranged from 0.476 to 0.98. Matrices derived from both isozyme and RAPD data were used to construct UPGMA dendrograms. In the tree obtained from Nei's genetic distance, based on allozyme frequencies, accessions belonging toV. marinassp.oblongawere mixed withV. luteolaaccessions; on the other hand, the twoV. marinassp.marinaclustered separately, with oneV. luteola.The dendrogram derived from RAPD data showed three main groups corresponding to the three taxa analysed. Moreover, according to these data,V. marinassp.oblongais more closely related toV. luteolathan toV. marinassp.marina.Copyright 1997 Annals of Botany Company

Vigna luteola(Jacq.) Benth.; Vigna marina(Burm.) Merrill; isozymes; RAPDs; genetic relationships; genetic variation


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