Annals of Botany 80: 787-793, 1997
© 1997 Annals of Botany Company
Identification of the Genomic Constitution ofMusaL. Lines (Bananas, Plantains and Hybrids) Using Molecular Cytogenetics
,Karyobiology Group, Department of Cell Biology, John Innes Centre, Colney Lane, Norwich, NR4 7UH, U.K. Department of Botany, University of Port Harcourt, P.M.B. 5323, Port Harcourt, Rivers State, Nigeria International Institute of Tropical Agriculture, c/o LW Lambourne & Co, Carolyn House, 26 Dingwall Road, Croydon, CR9 3EE, U.K.
June 30, 1997 ; July 22, 1997 . August 20, 1997 .
The genomic constitutions of someMusaL. lines (bananas, plantains and artificial hybrids) were identified using molecular cytogenetic techniques. Double targetin situDNA:DNA hybridization to chromosome spreads using as probes, total genomic DNA isolated from diploidMusalines of known AA (labelled with biotin-11-dUTP) and BB (labelled with digoxigenin-11-dUTP) genome constitution was carried out. The use of 60% acetic acid combined with heating over a flame gave high quality chromosome spreads free of cytoplasm forin situhybridization. Total genomic A DNA labelled broad centromeric regions of all 22 chromosomes of the diploid line, Calcutta 4 (M. acuminataColla. ssp.burmanniccoides; A genome) with some chromosomes showing stronger hybridization. Labelled DNA from the B genome hybridized strongly to the centromeric regions of all 22 chromosomes of Butohan 2 (M. balbisianaColla; B genome). The two satellited chromosomes of genome B labelled strongly with genomic A DNA.In situhybridization of labelled A and B genomic DNA to metaphase chromosomes of triploid AAB and ABB cultivars discriminated between A and B genome chromosomes. The plantains Agbagba, Obino l'Ewai and Mbi Egome showed 22 genome A and 11 genome B chromosomes while the cooking bananas Bluggoe and Fougamou showed 11 genome A and 22 genome B chromosomes. Hybridization of labelled A and B genomic DNA to chromosomes of the hybrids showed that TMP2x 2829-62 has all 22 genome A chromosomes while TMPx 4698-1 has 33 genome A and 11 genome B chromosomes.In situhybridization of labelled total genomic DNA to chromosomes has immense potential for identification of chromosome origin and can be used to characterize cultivars and hybrids produced inMusabreeding.Copyright 1997 Annals of Botany Company
Genomicin situhybridization; banana; plantain; hybrids; plant breeding; genome organization; biodiversity
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