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Annals of Botany 81: 355-362, 1998
© 1998 Annals of Botany Company

Morphological Alterations and Root Nodule Formation inAgrobacterium rhizogenes-mediated Transgenic Hairy Roots of Peanut (Arachis hypogaeaL.)

YOKO AKASAKA, MASAHIRO MII and HIROYUKI DAIMON+,

Faculty of Horticulture, Chiba University, Matsudo, Chiba, 271, Japan College of Agriculture, Osaka Prefecture University, Sakai, Osaka, 593, Japan

August 21, 1997 ; September 24, 1997 . October 27, 1997 .

Transformed hairy roots were induced at the excised site of the epicotyl of dry mature seed of a Spanish type peanut(Arachis hypogaea)cv. Java 13 2 weeks after inoculation with a wild type strain ofAgrobacterium rhizogenes,MAFF-02-10266. Composite plants consisting of transformed roots with non-transformed shoots were cultured using pouches. Forty days after inoculation, the composite plant showed a root system with abundant root mass, more lateral branching and high fractal dimension compared to the control. No differences were observed in production of rosette-type root hairs or the cross sectional structure between transformed and non-transformed roots. The inoculation ofBradyrhizobiumsp. A2R1 strain to the composite plants led to the induction of transformed root nodules. These transformed root nodules showed production of leghaemoglobin in the bacterial zone and nitrogenase activity as assayed by C2H2reduction, and exhibited enlargement of the nodule cortex region andde novoroot formation from the nodule cortex.Copyright 1998 Annals of Botany Company

Agrobacterium rhizogenes;Arachis hypogaeaL.; composite plant; peanut; transformation; root nodule.


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