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Annals of Botany 81: 391-396, 1998
© 1998 Annals of Botany Company

Initiation and Morphogenesis of Groundnut (Arachis hypogaeaL.) Pods in Solution Culture

G. E. ZHARARE+,, F. P. C. BLAMEY§, and C. J. ASHER

Department of Agriculture, The University of Queensland, Brisbane, Qld, 4072, Australia

August 28, 1997 ; October 30, 1997 . November 11, 1997 .

A recently-developed solution culture technique was used to study the effects of aeration and calcium (Ca) on groundnut(Arachis hypogaeaL.) pod development. Two experiments were conducted with seven groundnut lines, TMV-2, Chico and A116L4 (Spanish), CBRR4 (Valencia), A125L25 (ValenciaxSpanish), and Shulamit Strain 1 (SH-1) and Virginia Brunch Strain 1 (VB-1) (Virginia). Plants were grown in a potting mix, and the attached gynophores cultured in darkened polycarbonate jars containing nutrient solution. Non-aeration of solution prevented pod development, but pods and seeds of all lines developed in aerated, darkened nutrient solutions (ionic strength approx. 9 mM). Normal pods and seeds were produced by TMV-2, Chico and CBRR4, but constricted pods developed in SH-1 and VB-1. A secondary gynophore developed between the basal and apical seed compartments in A116L4 and A125L25, and in VB-1 at high Ca (500–2500 µM) in solution. The secondary gynophores were similar to those produced in otherArachisspp. but not usually found in cultivated forms ofA. hypogaea.Septate and non-septate hairs developed on submerged gynophores and pods, but were sparse on those of SH-1 and VB-1. The magnitude of the effects of aeration and Ca concentration on pod initiation and morphogenesis differed in experiments conducted in summer and winter and among the lines tested.Copyright 1998 Annals of Botany Company

Arachis hypogaeaL., calcium, groundnut, pod morphology, secondary gynophore, solution culture.


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