Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by RATH, P.
Right arrow Articles by KUMAR, P. P.
Right arrow Search for Related Content
PubMed
Right arrow Articles by RATH, P.
Right arrow Articles by KUMAR, P. P.
Agricola
Right arrow Articles by RATH, P.
Right arrow Articles by KUMAR, P. P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 82: 61-65, 1998
© 1998 Annals of Botany Company

Phylogenetic Analysis of Dipterocarps Using Random Amplified Polymorphic DNA Markers

PRIYADARSHINI RATH, G. RAJASEGER, CHONG JIN GOH and PRAKASH P. KUMAR+,

School of Biological Sciences, The National University of Singapore, 10 Kent Ridge Crescent, Singapore, 119260

November 10, 1997 ; December 23, 1997 . March 26, 1998 .

The phylogenetic relationships among 12 species belonging to three different genera (Shorea, HopeaandAnisoptera) of Dipterocarpaceae were studied using random amplified polymorphic DNA (RAPD) markers. A modified CTAB DNA extraction protocol was used to obtain tannin- and polysaccharide-free genomic DNA from mature leaves. Cluster analysis of data from six random primers placed the 12 species in three groups corresponding to their respective genera. Four distinct nodes ofShoreaspp. and two ofHopeaspp. could be identified.Anisoptera megistocarpaserved as an outgroup, and was unique when compared to the other genera examined. RAPD profiles of five individuals ofH. odoratawith six random primers were identical, suggesting that there is little intraspecific variation in this species. The RAPD technique can thus be successfully applied for the study of phylogenetic relationships of this important group of tropical timber trees.Copyright 1998 Annals of Botany Company

Dipterocarpaceae,Anisopteraspp.,Hopeaspp.,Shoreaspp., RAPD markers, tropical timber trees.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.