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Annals of Botany 82: 337-345, 1998
© 1998 Annals of Botany Company

The Cellular Pathway of Short-distance Transfer of Photosynthates and Potassium in the Elongating Stem ofPhaseolus vulgarisL. A Physiological Assessment

ROBYN M. WOOD, JOHN W. PATRICK+, and CHRISTINA E. OFFLER

Department of Biological Sciences, The University of Newcastle, NSW, 2308, Australia

February 3, 1998 ; April 6, 1998 . May 21, 1998 .

Plasmolytic disruption of plasmodesmata interconnecting metaphloem sieve element-companion cell complexes with small and large phloem parenchyma cells in the elongating region of internode 2 ofPhaseolus vulgarisL. seedlings did not affect accumulation of phloem-imported14C-photosynthates and86rubidium. The membrane-impermeant dye, 5(6) carboxyfluorescein, loaded into leaf phloem as the membrane-permeant diacetate ester, was found not to move radially out of the importing sieve elements in the internode elongation region. In contrast, the apoplasmic tracer, Calcuofluor White, rapidly moved laterally throughout all tissues of the elongation zone. Hexoses, sucrose and potassium were identified as the main osmotica in internode apoplasmic sap. Label asymmetry in [14C](fructosyl)sucrose was retained on accumulation by excised stem segments. Uptake of [14C]sucrose and86rubidium by stem segments exhibited saturation kinetics. Sucrose uptake was inhibited by the slowly penetrating sulphydryl reagent, para-chloromercuribenzenesulphonic acid.In vitrorates of sucrose uptake, at apoplasmic concentrations, corresponded to its predictedin vivorate of delivery to the stem ground tissues from mature sieve elements when respiratory losses were assumed to be confined to the stem phloem. For potassium, the total delivery rate could be accounted for by itsin vitrorate of uptake. Overall, it was concluded that radial transport, in the elongation zone of internode 2 ofPhaseolus vulgarisL. seedlings, follows an apoplasmic route from mature sieve elements to stem ground tissues.Copyright 1998 Annals of Botany Company

PhaseoluLes vulgaris, apoplasm, elongating stem, French bean, photosynthates, potassium, radial transfer, symplasm.


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