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Annals of Botany 82: 683-686, 1998
© 1998 Annals of Botany Company

COMMUNICATION

Interaction Between Cryopreservation, Rewarming Rate and Seed Humidification on the Germination of Two Spanish Endemic Species

M. ELENA GONZÁLEZ-BENITO, F. FERNÁNDEZ-LLORENTE and FÉLIX PÉREZ-GARCÍA

Dpto. de Biología Vegetal,, Escuela Universitaria de Ingeniería Técnica Agrícola, Universidad Politécnica de Madrid, Ciudad Universitaria, 28040, Madrid, Spain

February 9, 1998 ; April 30, 1998 . June 8, 1998 .

ABSTRACT

The effects of humidification, storage in liquid nitrogen (1 or 30 d) and rewarming rate on seed germination were studied in two Spanish endemics. Humidification resulted in higher germination percentages only in the species with hard covers, especially in slowly rewarmed seeds. In an experiment lasting 21 weeks, seeds stored in liquid nitrogen were removed for 10 min each week to mimic the withdrawal of samples from a seed bank; this had no effect on germination.Copyright 1998 Annals of Botany Company

Centaurea hyssopifolia,Limonium dichotomum, cryopreservation, cypsela, endemics, germination, humidification, seeds.


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