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Annals of Botany 82: 727-733, 1998
© 1998 Annals of Botany Company

In situMechanical Testing of Fully Hydrated Carrots (Daucus carota) in the Environmental SEM

B. L. THIEL+, and A. M. DONALD

Polymers and Colloids Group, Cavendish Laboratory, Department of Physics, Madingley Road, University of Cambridge, Cambridge, CB3 0HE, UK

March 11, 1998 ; July 16, 1998 .

We demonstrate the use of the Environmental Scanning Electron Microscope (ESEM) forin situobservation of mechanical tests on carrot (Daucus carota)parenchymal tissue. The ESEM tolerates several Torr of water vapour in the specimen chamber, thus allowing fully hydrated specimens to be examined at high resolution, but without preparation. Three tests were performed, involving slicing, tension and compression. The manner in which stress was distributed in the tissue, crack propagation and cell wall distortion were all observed in real time.Copyright 1998 Annals of Botany Company.

Environmental scanning electron microscopy, carrot,Daucus carota,mechanical testing.


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