Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by VALDEZ, M.
Right arrow Articles by CHRISTOU, P.
Right arrow Search for Related Content
PubMed
Right arrow Articles by VALDEZ, M.
Right arrow Articles by CHRISTOU, P.
Agricola
Right arrow Articles by VALDEZ, M.
Right arrow Articles by CHRISTOU, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 82: 795-801, 1998
© 1998 Annals of Botany Company

Transgenic Central American, West African and Asian Elite Rice Varieties Resulting from Particle Bombardment of Foreign DNA into Mature Seed-derived Explants Utilizing Three Different Bombardment Devices

M. VALDEZ, J. L. CABRERA-PONCE, D. SUDHAKAR, L. HERRERA-ESTRELLA and P. CHRISTOU

Escuela de Biologia, Universidad de Costa Rica, San Jose, Costa Rica Centro de Investigacion y de Estudios Avanzados del IPN, Departamento de Ingeniera Genetica de Plantas, Apdo. Postal 629, Irapuato, Guanajuato, Mexico John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, United Kingdom

June 5, 1998 ; July 4, 1998 . August 3, 1998 .

We developed a novel system based on particle bombardment for the transfer of foreign genes into eight important rice varieties grown in Central America, West Africa and Asia. Novel features of this improved protocol, which we believe will replace alternative systems currently utilizing immature embryos, embryogenic cell lines or suspension cultures include: (a) bombardment of target tissue 5 d after seed germination; (b) no requirement to select for embryogenic callus prior to bombardment; (c) high transformation frequencies (up to 14%); (d) ability to recover transgenic material at similar frequencies irrespective of bombardment devices used. Southern blot analysis and enzymatic assays in primary transformants and progeny confirmed the stable integration and expression of introduced transgenes in all engineered cultivars.Copyright 1998 Annals of Botany Company.

Oryza sativa, transgenic rice, particle bombardment, transformation.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Capell, L. Bassie, and P. Christou
Modulation of the polyamine biosynthetic pathway in transgenic rice confers tolerance to drought stress
PNAS, June 29, 2004; 101(26): 9909 - 9914.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Thu-Hang, L. Bassie, G. Safwat, P. Trung-Nghia, P. Christou, and T. Capell
Expression of a Heterologous S-Adenosylmethionine Decarboxylase cDNA in Plants Demonstrates That Changes in S-Adenosyl-L-Methionine Decarboxylase Activity Determine Levels of the Higher Polyamines Spermidine and Spermine
Plant Physiology, August 1, 2002; 129(4): 1744 - 1754.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
J. Jiang, S. D. Linscombe, J. Wang, and J. H. Oard
High Efficiency Transformation of U.S. Rice Lines from Mature Seed-Derived Calli and Segregation of Glufosinate Resistance under Field Conditions
Crop Sci., November 1, 2000; 40(6): 1729 - 1741.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.