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Annals of Botany 84: 329-335, 1999
© 1999 Annals of Botany Company

Ultrastructural Organization of Leaves of Transgenic Tobacco Overexpressing Histone H1 from Arabidopsis thaliana

JOANNA SLUSARCZYK, MARTA PRYMAKOWSKA-BOSAK, MARCIN PRZEWLOKA, ANDRZEJ JERZMANOWSKI and MIECZYSLAW KURAS

Department of Ecology and Environmental Protection, Pedagogical School, ul.Konopnickiej 15, Kielce, Poland Laboratory of Plant Molecular Biology, Warsaw University, ul. Pawinskiego 5a, Warsaw, Poland Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul.Pawinskiego 5a, 02-106, Warsaw, Poland Department of Plant Morphogenesis, Warsaw University, ul. Banacha 2, Warsaw, Poland

January 8, 1999 ; March 19, 1999 . May 13, 1999

We investigated the anatomical and ultrastructural features of transgenic tobacco plants that overexpressed a gene of histone H1 from Arabidopsis thaliana. The overexpression of the heterologous gene resulted in more than a 2.5-fold increase over the physiological level of the histone H1:DNA ratio in chromatin. H1-overexpressing plants had a distinct mutant phenotype characterized by dwarf appearance and severely hampered flowering. These changes were accompanied by extensive and unusual heterochromatinization of nuclei occurring in all leaf parenchymal cells but not in leaf epidermal cells. The observed anomalies in the growth rate and size of the cells and in nuclei/chloroplast proportions in histone H1-overexpressing plants suggest that the H1:DNA ratio can influence some specialized cellular functions involving the cytoskeleton, and nuclear/organellar interactions which are of importance for the normal development of a plant. Copyright 1999 Annals of Botany Company

Transgenic tobacco plant, histone H1 overexpression, heterochromatinization.


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