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Annals of Botany 85: 845-850, 2000
© 2000 Annals of Botany Company

Sectional Delineation of Sexual Tripsacum dactyloidesT. maizar Allotriploids

Yong G. Li, Chet L. Dewald+ and Victor A. Sokolov

USDA-ARS, Southern Plains Range Research Station, Woodward, Oklahoma, 73801, USA Institute of Cytology & Genetics, Novosibirsk, 630090, Russia

Received: 5 January 2000 ; Returned for revision: 2 February 2000 . Accepted: 23 February 2000

In recent studies it was determined that certain allotriploids produced from crosses of sexual diploidTripsacum dactyloides (2 n = 2 x = 36), sectionTripsacum and apomictic tetraploid T. maizar(2 n = 4x = 72), section Fasciculata reproduce sexually. Backcrossing progenies can be clearly classified to either sect. Tripsacum or sect. Fasciculata due to their distinctively different inflorescence phenotype. More importantly, the sectional delineation of the progeny shifted in the direction of the pollinator through genome substitution, which is not to be expected following just one generation of backcrossing in a normal breeding program. Our main objective was to investigate whether there were potential genetic differences between the genomes from sect. Tripsacum and sect. Fasciculata at the molecular level that correlate with such morphological shifts in these unusual sexual allotriploids. Cluster analysis based on randomly amplified polymorphic DNA (RAPD) profiles of these sexual allotriploids clearly indicated that substantial differences among many loci do exist between T. dactyloides and T. maizar. These results support the taxonomic classification of Tripsacum species into section Tripsacum and sectionFasciculata . Copyright 2000 Annals of Botany Company

Tripsacum allotriploid, sectional delineation, RAPD, inflorescence morphology


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