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Annals of Botany 85 (Supplement A): 133-139, 2000
© 2000 Annals of Botany Company

Molecular Genetics of Self-incompatibility in Brassica napus

N. Brugière 1, Y. Cui 1, Y. Bi 1, M. Arnoldo 2, L. Jackman 1, and S. J. Rothstein 3

1 Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario Canada, N1G 2W1
2 Pioneer Hi-Bred Production Ltd, Canola Research Station, Georgetown, Ontario, Canada, L7G 4S7
3 Department of Agronomic traits, Pioneer Hi-Bred International Inc., P.O. Box 1004, Johnston, Iowa, USA, 50131

Department of Agronomic Traits, Pioneer Hi-Bred International Inc., P.O. Box 1004, Johnston, Iowa, USA 50131. Fax +1 519 837 2075, brugiere{at}yahoo.com

Self-incompatibility (SI) is one of the mechanisms evolved by higher plants to promote outbreeding. In Brassica species, SI is controlled sporophytically by a single Mendelian genetic locus, the S-locus. Pollen rejection or acceptance is determined by the parental genotype, and pollen grains carrying the same allele as that present in the stigma are rejected. Among the genes identified so far from different S-haplotypes, the S-locus glycoprotein (SLG) and the S-locus receptor kinase (SRK) have been extensively studied. The current model of SI in Brassica postulates that a highly polymorphic pollen-borne ligand would be specifically recognized by SLG and/or SRK. This would activate a signal transduction pathway leading to rejection of self-pollen. We used a genomic approach in an attempt to identify a putative ligand gene. The S-locus regions of two functional Brassica napus S-haplotypes (910 and A14) were subcloned in Fosmid vectors and sequenced. New transcription units were identified by homology searches in the databases, gene prediction programs and polymerase chain reaction (PCR) amplification. The current study reports the structural features of both S-locus regions and a functional analysis using a transformation-based assay.

Sporophytic self-incompatibility, S-locus region sequence, transgenic plants

Submitted on September 3, 1999
Revised on October 20, 1999
Accepted on October 22, 1999


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M. Watanabe, K. Hatakeyama, Y. Takada, and K. Hinata
Molecular Aspects of Self-Incompatibility in Brassica Species
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