1 Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK
Fax +44 (0)1865 275805; James.Doughty{at}plant-sciences.ox.ac.uk
In Brassica, recognition and rejection of ‘self’ pollen is rapid, occurring at the stigmatic surface usually prior to pollen germination and is mediated by products of the S (incompatibility)-locus. Two polymorphic stigmatically-expressed S-locus genes, thought to participate in the perception of ‘self’ pollen have been characterized to date. One, the SRK (S-receptor kinase) encodes a transmembrane receptor kinase likely to be activated by the binding of an S-specific pollen-borne ligand. The other, SLG (S-locus glycoprotein), encodes a secreted cell wall glycoprotein whose role in self-incompatibility (SI) is less clear and increasingly questioned. The pollen coating in Brassica is known to carry the male determinant of SI as well as factors crucial for competent pollen-stigma interactions. Characterization of the coating has revealed the presence of several families of gametophytically expressed small cysteine-rich proteins (PCPs—pollen coat proteins). PCPs are strongly implicated in playing a central role in SI and other aspects of the pollen-stigma interaction. PCP-A (for PCP, class ‘A’) class proteins have specific affinities for stigmatic S- and S-related proteins and these, along with other recently characterized groups of PCPs, are abundant components of fractions having S-specific activity in pollination bioassays. The role of the pollen coating, and particularly PCPs and their expression patterns, will be discussed in the perspective of the pollen-stigma interaction and SI.
Self-incompatibility, pollen coat protein, pollen, PCP-A1, SRK, SLR1, SLG, Brassica oleracea, tryphine, recognition, stigma, signalling
Submitted on July 21, 1999
© 2000 Annals of Botany Company
Cysteine-rich Pollen Coat Proteins (PCPs) and their Interactions with Stigmatic S (Incompatibility) and S-Related Proteins in Brassica: Putative Roles in SI and Pollination
2 Oxford University Museum of Natural History, Parks Road, Oxford, OX1 3PW, UK
Revised on October 19, 1999
Accepted on October 27, 1999
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