Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Dowd, P. E.
Right arrow Articles by Kao, T.-H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Dowd, P. E.
Right arrow Articles by Kao, T.-H.
Agricola
Right arrow Articles by Dowd, P. E.
Right arrow Articles by Kao, T.-H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 85 (Supplement A): 87-93, 2000
© 2000 Annals of Botany Company

Use of Petunia inflata as a Model for the Study of Solanaceous Type Self-incompatibility

Peter E. Dowd 1, Andrew G. McCubbin 1, Xi Wang 1, Joseph A. Verica 1, Tatsuya Tsukamoto 2, Toshio Ando 2, and Teh-Hui Kao 1

1 Department of Biochemistry and Molecular Biology, 403 Althouse Laboratory, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
2 Faculty of Horticulture, Chiba University, 648 Matsudo, Chiba Prefecture 271-8510, Japan

Fax +1 814-863-9416, txk3{at}psu.edu

Petunia inflata has been used as a model to study solanaceous type self-incompatibility (SI), an RNase-mediated self/non-self recognition mechanism. Pistil proteins that co-segregate with S-alleles (termed S-proteins or S-RNases) were identified, and their role in SI was demonstrated by gain-of-function and loss-of-function experiments. Several lines of evidence strongly suggest that the S-locus contains another gene, termed the pollen S-gene, whose allelic products presumably interact with S-RNases to trigger the SI response. Thus, ultimate understanding of the molecular/biochemical basis of SI requires the identification of the pollen S-gene. A number of approaches that have been used to accomplish this objective are discussed. Differential display and subtractive hybridization have yielded the most promising results: cDNAs corresponding to 13 pollen-expressed genes that lie within 1 cM of the S-RNase gene have been identified. They will be used as markers to identify large S-linked genomic fragments which will then be examined for the presence of the pollen S-gene by plant transformation.

Petunia inflata, pollen-pistil interactions, S-linked cDNA markers, S-RNases, self-incompatibility

Submitted on July 28, 1999
Revised on September 9, 1999
Accepted on October 22, 1999


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GeneticsHome page
V. Llaurens, L. Gonthier, and S. Billiard
The Sheltered Genetic Load Linked to the S Locus in Plants: New Insights From Theoretical and Empirical Approaches in Sporophytic Self-Incompatibility
Genetics, November 1, 2009; 183(3): 1105 - 1118.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
N. Takebayashi, P. B. Brewer, E. Newbigin, and M. K. Uyenoyama
Patterns of Variation Within Self-Incompatibility Loci
Mol. Biol. Evol., November 1, 2003; 20(11): 1778 - 1794.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Wang, X. Wang, A. L. Skirpan, and T.-h. Kao
S-RNase-mediated self-incompatibility
J. Exp. Bot., January 1, 2003; 54(380): 115 - 122.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. Glemin, T. Bataillon, J. Ronfort, A. Mignot, and I. Olivieri
Inbreeding Depression in Small Populations of Self-Incompatible Plants
Genetics, November 1, 2001; 159(3): 1217 - 1229.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.