Annals of Botany 86: 201-204, 2000
© 2000 Annals of Botany Company
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Molecular Cytogenetic Localization and Characterization of 5S and 25S rDNA Loci for Chromosome Identification in Oilseed Rape (Brassica napus L.)
Institut für Pflanzenbau und Pflanzenzüchtung, Justus-Liebig-Universität Giessen, IPZ, Heinrich-Buff-Ring 2732, 35392, Giessen, Germany
Received: 4 January 2000 ; Returned for revision: 15 February 2000 . Accepted: 16 March 2000
ABSTRACT
Chromosome identification in oilseed rape (Brassica napus L.) is extremely difficult using conventional cytogenetic techniques because amphidiploid Brassica species possess numerous very small chromosomes with few cytogenetic landmarks. In combination with methods for improved chromosome preparations, we used a simplified fluorescencein situ hybridization (FISH) technique to localize simultaneously the gene families coding for 5S and 25S rDNA in B. napus. The resulting hybridization patterns enabled ten of the 19 oilseed rape chromosome pairs to be unequivocally identified. Copyright 2000 Annals of Botany Company
Brassica napus, oilseed rape, rDNA, molecular cytogenetics, FISH, chromosome identification
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