Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Katsiotis, A.
Right arrow Articles by Heslop-Harrison, J. S.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Katsiotis, A.
Right arrow Articles by Heslop-Harrison, J. S.
Agricola
Right arrow Articles by Katsiotis, A.
Right arrow Articles by Heslop-Harrison, J. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Annals of Botany 86: 1135-1142, 2000
© 2000 Annals of Botany Company

Repetitive DNA, Genome and Species Relationships in Avena and Arrhenatherum(Poaceae)

A. Katsiotis+, M. Loukas and J. S. Heslop-Harrison

Laboratory of Genetics, Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, GR-118 55, Athens, Greece Karyobiology Group, John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK

Received: 1 February 2000 ; Returned for revision: 13 June 2000 . Accepted: 21 August 2000

Repetitive sequences have been widely used for examining genome and species relationships by in situ and Southern hybridization. In the present study, double-stranded DNA sequences, from denatured DNA reannealed to Cot = 1, from Avena strigosa(2 n = 2x = 14; A genome; referred to as CotA) and Avena sativa(2n = 6 x = 42; ACD genome; referred to as CotACD) were isolated with a hydroxyapatite column, and were used for in situ hybridization on hexaploid A. sativa chromosomes. Probe CotACD labelled all chromosomes evenly throughout their length at the same intensity. Probe CotA labelled the 28 A and D genome chromosomes strongly and the 14 C genome chromosomes weakly. Three cloned repetitive sequences, pAvKB9 (126 bp), pAvKB26 (223 bp) and pAvKB32 (721 bp) were characterized in the A, B, C and D Avena genomes and the genus Arrhenatherum using molecular and cytological methods. Clones pAvKB9 and pAvKB26 were absent from the Avena C genome, while both could identify the presence of the D genome by Southern hybridization. In situ hybridization to diploid and tetraploid Avena species revealed that the probes showed a dispersed genomic organization and that they are present on both arms of all chromosomes. These sequences were excluded from areas where tandem repeats, such as rRNA genes and telomeres, are present. These results indicate the close relationship between A and D genomes and the presence of common DNA sequences between A and C Avena genomes. All three clones hybridized to Southern blots containingArrhenatherum digested genomic DNA, indicating Arrhenatherum’s close affinity to A, B and D Avena genomes. Copyright 2000 Annals of Botany Company

Cereals, DNA, hydroxyapatite, in situ hybridization, oats, reassociation kinetics, repetitive DNA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Crop Sci.Home page
C. C. Mitchell, S. E. Parkinson, T. J. Baker, and E. N. Jellen
C-Banding and Localization of 18S-5.8S-26S rDNA in Tall Oatgrass Species
Crop Sci., January 1, 2003; 43(1): 32 - 36.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.