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Annals of Botany 87: 125-129, 2001
© 2001 Annals of Botany Company

Functional Characterization of Two Ripening-related Sucrose Transporters from Grape Berries

K. Manning+, C. Davies, H. C. Bowen and P. J. White

Department of Plant Genetics and Biotechnology, Horticulture Research International, Wellesbourne, Warwick, CV35 9EF, UK Commonwealth Scientific and Industrial Research Organization, Plant Industry, Horticulture Research Unit, P.O. Box 350, Glen Osmond, South Australia, 5064, Australia

Received: 18 August 2000 ; Returned for revision: 21 September 2000 . Accepted: 3 October 2000

The ripening of grape (Vitis vinifera L.) berries is associated with a large accumulation of glucose and fructose in the vacuoles of the fruit cells. These hexoses are derived from sucrose, which is released from the phloem and may be taken up by parenchyma cells prior to hydrolysis. We have expressed two putative ripening-related sucrose transporters from grape berries, VvSUC11 (synonymous with VvSUT1) and VvSUC12, in an invertase deficient yeast strain to characterize their transport activities. Sucrose was taken up by yeast transformed with either transporter at an optimum pH of <4.5 and with a Michaelis constant (Km) of 0.9–1.4 m M. The uptake of sucrose through VvSUC11 and VvSUC12 was inhibited by protonophores and by vanadate. This is consistent with an active uptake mechanism involving proton cotransport, typical of sucrose/H+symporters. The transporters from grape berries were functionally similar to Scr1, a sucrose transporter from Ricinus cotyledons. It is likely that in grape berries VvSUC11 and VvSUC12 facilitate the loading of sucrose from the apoplast into the parenchyma cells. Copyright 2001 Annals of Botany Company

Fruit, grape berries, plasma membrane, sugars, sucrose transporters, Vitis vinifera


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